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Travertine deposits preserve an invaluable record of both ancient and modern fluid flow. The goal of this study is to reconstruct spatial and temporal patterns in travertine deposition associated with tectonic and climatic controls along the Lucero Uplift in New Mexico, USA. Uranium‐series ages of travertine deposits in the Lucero Uplift range from 0.94 ± 0.01 to 592 ± 110 ka, indicating that travertine formation has been episodically active since at least ∼600 ka. We find minimal evidence to attribute glacial and interglacial cycles to travertine formation in the Lucero Uplift. δ¹³C values in travertine deposits range from 2‰ to 9‰ (Vienna Pee Dee Belemnite), δ¹⁸O values range from 21‰ to 25‰ (Vienna Standard Mean Ocean Water). Positive correlation between travertine δ¹³C and δ¹⁸O values indicate travertine formation is closely associated with various degrees of CO₂degassing.⁸⁷Sr/⁸⁶Sr values in travertine deposits range from 0.714 to 0.717 and (²³⁴U/²³⁸U)_ivalues exhibit a remarkably wide range from 3.6 to 9.3, indicative of fluid‐rock interaction during deep crustal circulation in more radiogenic basement rocks. Reconstructed δ¹³C, δ¹⁸O, and (²³⁴U/²³⁸U)_ivalues in the inferred deep fluid sources showed systematic variations with travertine formation ages, while⁸⁷Sr/⁸⁶Sr values remain relatively constant. Based on dating of undeformed travertine deposits, which overlie tilted Santa Fe Group units, and high (²³⁴U/²³⁸U)_iwe infer that the Santa Fe fault has not produced a ground‐rupturing earthquake within the last 490 ± 52 to 592 ± 110 ka (2σ). Our study suggest that travertine formation is driven by fluid flow facilitated by tectonic and mantle structures.